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Literature summary extracted from

  • Akram, M.; Dietl, A.; Mueller, M.; Barends, T.R.M.
    Purification of the key enzyme complexes of the anammox pathway from DEMON sludge (2021), Biopolymers, 112, e23428 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.2.7 sitting drop vapor diffusion method Candidatus Brocadia fulgida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.8 hydroxylamine
-
 Candidatus Brocadia fulgida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.8 0.0076
-
 hydrazine at pH 7.0 and 35°C Candidatus Brocadia fulgida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.8 200700
-
 gel filtration Candidatus Brocadia fulgida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.7 nitric oxide + ammonium + 3 ferrocytochrome c Candidatus Brocadia fulgida
-
 hydrazine + H2O + 3 ferricytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.7 Candidatus Brocadia fulgida
-
-
-
1.7.2.8 Candidatus Brocadia fulgida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.7 Q Sepharose column chromatography, hydroxyapatite column chromatography and Superose 6 gel filtration Candidatus Brocadia fulgida
1.7.2.8 Q Sepharose column chromatography, hydroxyapatite column chromatography and Superose 6 gel filtration Candidatus Brocadia fulgida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.7 nitric oxide + ammonium + 3 ferrocytochrome c
-
 Candidatus Brocadia fulgida hydrazine + H2O + 3 ferricytochrome c
-
 ?
1.7.2.8 hydrazine + 4 bovine heart ferricytochrome c
-
 Candidatus Brocadia fulgida N2 + 4 bovine heart ferrocytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.7 heterotrimer
-
 Candidatus Brocadia fulgida
1.7.2.8 trimer 3 * 66900, calculated from amino acid sequence Candidatus Brocadia fulgida

Synonyms

EC Number Synonyms Comment Organism
1.7.2.7 HZS
-
 Candidatus Brocadia fulgida
1.7.2.8 HDH
-
 Candidatus Brocadia fulgida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.8 1.7
-
 hydrazine at pH 7.0 and 35°C Candidatus Brocadia fulgida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.2.8 220
-
 hydrazine at pH 7.0 and 35°C Candidatus Brocadia fulgida